Effects of Isotretinoin on Meibomian Glands

Effects of age and dysfunction on human meibomian glands.

OBJECTIVE To identify age-related changes in human meibomian glands that may be associated with meibomian gland dysfunction (MGD). METHODS Excess eyelid tissue from 36 patients (age range, 18-95 years, 19 female, 17 male) who underwent canthoplasty procedures were used. Dermatologic history, age, and presence of MGD were recorded. Samples were frozen, sectioned, and stained with specific anti...

Congenital deficiency of meibomian glands.

A 16-year-old girl presented with contact lens intolerance. She was found to have a marked deficiency of meibomian glands in the upper lids and almost total absence in the lower lids. Evidence of tear film instability was found and attributed to deficient lid oil production. A daily wear soft contact lens was later fitted and tolerated.

Historical overview of imaging the meibomian glands

The development of meibomian glands in mice

PURPOSE The purpose of this study was to characterize the natural history of meibomian gland morphogenesis in the mouse. METHODS Embryonic (E) and post natal (P) C57Bl/6 mouse pups were obtained at E18.5, P0, P1, P3, P5, P8, P15, and P60. Eyelids were fixed and processed for en bloc staining with Phalloidin/DAPI to identify gland morphogenesis, or frozen for immunohistochemistry staining with...

Transcriptome analysis of aging mouse meibomian glands

PURPOSE Dry eye disease is a common condition associated with age-related meibomian gland dysfunction (ARMGD). We have previously shown that ARMGD occurs in old mice, similar to that observed in human patients with MGD. To begin to understand the mechanism underlying ARMGD, we generated transcriptome profiles of eyelids excised from young and old mice of both sexes. METHODS Male and female C5...